Prosthecomicrobium and Ancalomicrobium Medium

[所属分类:培养基配方] [发布时间:2021-7-14] [发布人:网站管理员2] [阅读次数:] [返回]

Prosthecomicrobium and Ancalomicrobium Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per 1030.0mL:

Ammonium sulfate .....................................................................0.25g
Glucose .......................................................................................0.25g
Na 2 HPO 4 ..................................................................................71.0mg
Modified Hutner’s basal salts ..................................................20.0mL
Vitamin solution.......................................................................10.0mL
Modified Hutner’s Basal Salts:
Composition per liter:
MgSO 4 ·7H 2 O..............................................................................29.7g
Nitrilotriacetic acid.....................................................................10.0g
CaCl 2 ·2H 2 O................................................................................3.34g
FeSO 4 ·7H 2 O ............................................................................99.0mg
Ammonium molybdate............................................................9.25mg
Metals “44”..............................................................................50.0mL
Preparation of Modified Hutner’s Basal Salts:  Dissolve the
nitrilotracetic acid first and neutralize the solution with KOH. Add oth-
er components and adjust the pH to 7.2 with KOH or H 2 SO 4 . There
may be a slight precipitate. Store at 5°C.
Metals “44”
Composition per liter:
ZnSO 4 ·7H 2 O.................................................................................1.1g
FeSO 4 ·7H 2 O .................................................................................0.5g
CuSO 4 ·5H 2 O...............................................................................0.04g
EDTA..........................................................................................0.25g
MnSO 4 ·7H 2 O............................................................................0.154g
Co(NO 3 ) 2 ·6H 2 O........................................................................0.025g
Na 2 B 4 O 7 ·10H 2 O.......................................................................0.018g
Preparation of Metals “44”:  Add components to distilled/deion-
ized water and bring volume to 100.0mL. Mix thoroughly. Autoclave
for 15 min at 15 psi pressure–121°C. Add aseptically to sterile modi-
fied Hutner’s basal salts solution.
Vitamin Solution:
Composition per liter:
Cyanocobalamin ......................................................................10.0mg
Pyridoxine·HCl........................................................................10.0mg
Thiamine·HCl ..........................................................................10.0mg
Calcium  DL -pantothenate...........................................................5.0mg
Nicotinamide..............................................................................5.0mg
Biotin .........................................................................................2.0mg
Folic acid....................................................................................2.0mg
Preparation of Vitamin Solution:  Add components to distilled/
deionized water and bring volume to 1.0L. Mix thoroughly. Filter ster-
ilize.
Preparation of Medium: Add components, except modified Hut-
ner’s basal salts solution and vitamin solution, to distilled/deionized
water and bring volume to 970.0mL. Mix thoroughly. Autoclave for 15
min at 15 psi pressure–121°C. Aseptically add 20.0mL of modified
Hutner’s basal salts solution and 10.0mL of sterile vitamin solution.
Mix thoroughly. Aseptically distribute into sterile tubes or flasks.
Use: For the cultivation of Prosthecomicrobium enhydrum and Pros-
thecomicrobium pneumaticum.