Proteose No. 3 Agar

[所属分类:培养基配方] [发布时间:2021-7-14] [发布人:网站管理员2] [阅读次数:] [返回]

Proteose No. 3 Agar

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per 1010.0mL:

Proteose peptone No. 3...............................................................20.0g
Agar ............................................................................................15.0g
Na 2 HPO 4 .......................................................................................5.0g
NaCl..............................................................................................5.0g
Glucose .........................................................................................0.5g
Hemoglobin solution..............................................................500.0mL
Supplement A ..........................................................................10.0mL
pH 7.3 ± 0.2 at 25°C
Source:  This medium is available as a premixed powder from BD Di-
agnostic Systems.
Hemoglobin Solution:
Composition per 500.0mL:
Hemoglobin ................................................................................10.0g
Preparation of Hemoglobin Solution:  Add hemoglobin to dis-
tilled/deionized water and bring volume to 500.0mL. Mix thoroughly.
Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C.
Supplement A:
Composition per 10.0.mL:
Supplement A contains yeast concentrate with Crystal Violet.
Preparation of Supplement A:  Add components to distilled/de-
ionized water and bring volume to 10.0mL. Mix thoroughly. Filter ster-
ilize.
Preparation of Medium: Add components, except hemoglobin so-
lution and supplement A, to distilled/deionized water and bring volume
to 500.0mL. Mix thoroughly. Gently heat and bring to boiling. Auto-
clave for 15 min at 15 psi pressure–121°C. Cool to 50°–60°C. Asepti-
cally add 500.0mL of sterile hemoglobin solution and 10.0mL of
sterile supplement A. Mix thoroughly. Pour into sterile Petri dishes or
distribute into sterile tubes.
Use: For the isolation and cultivation of Neisseria species, Hemophi-
lus species, and other fastidious bacteria. For the cultivation and main-
tenance of Escherichia coli.