Prototheca Isolation Agar (PIM)

[所属分类:培养基配方] [发布时间:2021-7-14] [发布人:网站管理员2] [阅读次数:] [返回]

Prototheca Isolation Agar
(PIM)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................20.0g
Glucose .......................................................................................10.0g
Potassium hydrogen phthalate....................................................10.0g
NaOH............................................................................................0.9g
NH 4 Cl ...........................................................................................0.3g
5-Fluorocytosine.........................................................................0.25g
KH 2 PO 4 .........................................................................................0.2g
MgSO 4 ..........................................................................................0.1g
Thiamine·HCl ...........................................................................0.001g
pH 5.1 ± 0.1 at 25°C
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 5.1.
Gently heat and bring to boiling. Distribute into tubes or flasks. Auto-
clave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes
or leave in tubes.
Use: For the cultivation of Prototheca moriformis and Prototheca
ulmea.