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Pseudomonas aeruginosa Agar (PA Agar) (m–PA Agar) (m-Pseudomonas aeruginosa Agar)
[所属分类:培养基配方] [发布时间:2021-7-14] [发布人:网站管理员2] [阅读次数:] [返回]
Pseudomonas aeruginosa Agar
(PA Agar)
(m–PA Agar)
(m-Pseudomonas aeruginosa Agar)
Na 2 S 2 O 3 ........................................................................................6.8g
L- Lysine·HCl.................................................................................5.0g
NaCl..............................................................................................5.0g
Xylose...........................................................................................2.5g
Yeast extract..................................................................................2.0g
Lactose........................................................................................1.25g
Sucrose........................................................................................1.25g
Ferric ammonium citrate...............................................................0.8g
Sulfapyridine.............................................................................0.176g
Cycloheximide............................................................................0.15g
Phenol Red..................................................................................0.08g
Nalidixic acid............................................................................0.037g
Kanamycin.................................................................................8.5mg
pH 7.1 ± 0.2 at 25°C
Caution: Cycloheximide is toxic. Avoid skin contact or aerosol for-
mation and inhalation.
Preparation of Medium: Add components—except sulfapyridine,
cycloheximide, nalidixic acid, and kanamycin—to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 6.5. Au-
toclave for 15 min at 15 psi pressure–121°C. Cool to 55°–60°C. Read-
just pH to 7.1. Aseptically add the sulfapyridine, cycloheximide,
nalidixic acid, and kanamycin. Mix thoroughly. Pour into 50mm ×
12mm Petri dishes in 3.0mL volumes.
Use: For the cultivation and estimation of numbers of Pseudomonas
aeruginosa in water by the membrane filter method.
(PA Agar)
(m–PA Agar)
(m-Pseudomonas aeruginosa Agar)
山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com
Composition per liter:
Agar............................................................................................15.0gNa 2 S 2 O 3 ........................................................................................6.8g
L- Lysine·HCl.................................................................................5.0g
NaCl..............................................................................................5.0g
Xylose...........................................................................................2.5g
Yeast extract..................................................................................2.0g
Lactose........................................................................................1.25g
Sucrose........................................................................................1.25g
Ferric ammonium citrate...............................................................0.8g
Sulfapyridine.............................................................................0.176g
Cycloheximide............................................................................0.15g
Phenol Red..................................................................................0.08g
Nalidixic acid............................................................................0.037g
Kanamycin.................................................................................8.5mg
pH 7.1 ± 0.2 at 25°C
Caution: Cycloheximide is toxic. Avoid skin contact or aerosol for-
mation and inhalation.
Preparation of Medium: Add components—except sulfapyridine,
cycloheximide, nalidixic acid, and kanamycin—to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 6.5. Au-
toclave for 15 min at 15 psi pressure–121°C. Cool to 55°–60°C. Read-
just pH to 7.1. Aseptically add the sulfapyridine, cycloheximide,
nalidixic acid, and kanamycin. Mix thoroughly. Pour into 50mm ×
12mm Petri dishes in 3.0mL volumes.
Use: For the cultivation and estimation of numbers of Pseudomonas
aeruginosa in water by the membrane filter method.
