Pseudomonas Basal Mineral Medium

[所属分类:培养基配方] [发布时间:2021-7-15] [发布人:网站管理员2] [阅读次数:] [返回]

Pseudomonas Basal Mineral Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

K 2 HPO 4 .......................................................................................12.5g
KH 2 PO 4 .........................................................................................3.8g
(NH 4 ) 2 SO 4 .....................................................................................1.0g
MgSO 4 ·7H 2 O................................................................................0.1g
Carbon source (0.8M solution) ..............................................100.0mL
Trace elements solution .............................................................5.0mL
pH 7.2 ± 0.2 at 25°C
Trace Elements Solution:
Composition per liter:
H 3 BO 3 .......................................................................................0.232g
ZnSO 4 ·7H 2 O.............................................................................0.174g
FeSO 4 (NH 4 ) 2 SO 4 ·6H 2 O............................................................0.116g
CoSO 4 ·7H 2 O.............................................................................0.096g
(NH 4 ) 6 Mo 7 O 24 ·4H 2 O................................................................0.022g
CuSO 4 ·5H 2 O..............................................................................8.0mg
MnSO 4 ·4H 2 O.............................................................................8.0mg
Preparation of Trace Elements Solution: Add components to
distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Carbon Source:
Composition per 100.0mL:
Glucose .......................................................................................14.4g
Preparation of Carbon Source: Add glucose to distilled/deion-
ized water and bring volume to 100.0mL. Mix thoroughly. Filter ster-
ilize. Other carbon sources may replace glucose. Prepare 0.8M carbon
source solution.
Preparation of Medium: Add components, except carbon source,
to distilled/deionized water and bring volume to 900.0mL. Mix thor-
oughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi
pressure–121°C. Cool to 45°–50°C. Aseptically add 100.0mL of sterile
carbon source. Mix thoroughly. Aseptically distribute into sterile tubes
or flasks.
Use:  For the cultivation and differentiation of Pseudomonas species
based on their ability to grow on different carbon sources.