Pseudomonas CFC Agar

[所属分类:培养基配方] [发布时间:2021-7-15] [发布人:网站管理员2] [阅读次数:] [返回]

Pseudomonas CFC Agar

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter :

Pancreatic digest of gelatin.........................................................16.0g
Agar............................................................................................ 11.0g
Pancreatic digest of casein..........................................................10.0g
K 2 SO 4 .........................................................................................10.0g
MgCl 2 ·6H 2 O .................................................................................1.4g
CFC selective supplement .......................................................10.0mL
Glycerol ...................................................................................10.0mL
pH 7.1 ± 0.2 at 25°C
Source:  This medium is available as a premixed powder from Oxoid
Unipath.
CFC Selective Supplement:
Composition per 10.0mL:
Cephaloridine..............................................................................0.05g
Fucidin........................................................................................0.01g
Cetrimide....................................................................................0.01g
Preparation of CFC Selective Supplement: Add components to
distilled/deionized water and bring volume to 10.0mL. Mix thorough-
ly. Filter sterilize.
Preparation of Medium: Add components, except CFC selective
supplement, to distilled/deionized water and bring volume to 990.0mL.
Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min
at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add sterile
CFC selective supplement. Mix thoroughly. Pour into sterile Petri dish-
es or distribute into sterile tubes.
Use: For the selective isolation and cultivation of Pseudomonas spe-
cies.