Pseudomonas Medium (ATCC Medium 609)

[所属分类:培养基配方] [发布时间:2021-7-15] [发布人:网站管理员2] [阅读次数:] [返回]

Pseudomonas Medium
(ATCC Medium 609)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................15.0g
K 2 HPO 4 .......................................................................................8.71g
Nitrilotriacetic acid.....................................................................1.91g
Na 2 SO 4 ........................................................................................0.57g
MgSO 4 ........................................................................................0.25g
FeSO 4 .........................................................................................0.5mg
Ca(NO 3 ) 2 ....................................................................................0.5mg
pH 6.5 ± 0.2 at 25°C
Preparation of Medium: Add nitrilotriacetic acid to approximately
500.0mL of distilled/deionized water. Mix thoroughly. Adjust pH to
6.5. Add remaining components. Bring volume to 1.0L with distilled/
deionized water. Gently heat and bring to boiling. Distribute into tubes
or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Pour into
sterile Petri dishes or leave in tubes.
Use:  For the cultivation and maintenance of Pseudomonas species.