Pseudomonas Medium I

[所属分类:培养基配方] [发布时间:2021-7-15] [发布人:网站管理员2] [阅读次数:] [返回]

Pseudomonas Medium I

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Pancreatic digest of casein..........................................................10.0g
Yeast extract................................................................................10.0g
Glucose.........................................................................................5.0g
K 2 HPO 4 .........................................................................................5.0g
Salts solution..............................................................................5.0mL
Salts Solution:
Composition per 100.0mL:
MgSO 4 ·4H 2 O................................................................................4.0g
FeSO 4 ............................................................................................0.2g
MnSO 4 ·4H 2 O................................................................................0.2g
NaCl..............................................................................................0.2g
Preparation of Salts Solution: Add components to distilled/deion-
ized water and bring volume to 100.0mL. Mix thoroughly.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes
or flasks. Autoclave for 15 min at 15 psi pressure–121°C.
Use:  For the cultivation and maintenance of Bacillus sphaericus.