Pseudomonas syngii Medium

[所属分类:培养基配方] [发布时间:2021-7-16] [发布人:网站管理员2] [阅读次数:] [返回]

Pseudomonas syngii Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar............................................................................................15.0g
Acid casein hydrolysate................................................................7.5g
Sucrose..........................................................................................2.0g
MgSO 4 ·7H 2 O.........................................................................250.0mg
K 2 HPO 4 ..................................................................................500.0mg
Ammonium ferricitrate solution..............................................20.0mL
Ammonium Ferricitrate Solution:
Composition per 20.0mL:
Ammonium ferricitrate...............................................................0.25g
Preparation of Ammonium Ferricitrate Solution: Add ammo-
nium ferricitrate to distilled/deionized water and bring volume to
20.0mL. Mix thoroughly. Filter sterilize.
Preparation of Medium: Add components, except ammonium fer-
ricitrate solution, to distilled/deionized water and bring volume to
980.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave
for 15 min at 15 psi pressure–121°C. Cool to 50°–55°C. Aseptically
add 20.0mL of sterile ammonium ferricitrate solution. Mix thoroughly.
Pour into sterile Petri dishes or distribute into sterile tubes.
Use: For the cultivation and maintenance of Pseudomonas synygii.