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Purple Broth with Sodium Chloride (Purple Carbohydrate Broth with NaCl) (BAM M130)

[所属分类:培养基配方] [发布时间:2021-7-16] [发布人:网站管理员2] [阅读次数:] [返回]

Purple Broth with Sodium Chloride
(Purple Carbohydrate Broth with NaCl)
(BAM M130)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Proteose peptone No. 3...............................................................10.0g
NaCl..............................................................................................5.0g
Beef extract...................................................................................1.0g
Bromcresol Purple......................................................................0.02g
Carbohydrate solution..............................................................20.0mL
pH 6.8 ± 0.2 at 25°C
Source: This medium is available as a premixed powder from BD Di-
agnostic Systems.
Carbohydrate Solution:
Composition per 20.0mL:
Carbohydrate...............................................................................10.0g
Preparation of Carbohydrate Solution: Add carbohydrate to
distilled/deionized water and bring volume to 20.0mL. For expensive
carbohydrates, 5.0g may be used instead of 10.0g. Mix thoroughly. Fil-
ter sterilize.
Preparation of Medium: Add components, except carbohydrate
solution, to distilled/deionized water and bring volume to 980.0mL.
Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes
in 9.8mL volumes. Autoclave for 15 min at 15 psi pressure–121°C.
Cool to 25°C. Aseptically add 0.2mL of sterile carbohydrate solution
to each tube. Mix thoroughly.
Use: For the preparation of carbohydrate media used in fermentation
studies for the identification of bacteria, especially members of the En-
terobacteriaceae. Bacteria that can ferment the carbohydrate turn the
medium yellow.

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Purple Broth with Sodium Chloride (Purple Carbohydrate Broth with NaCl) (BAM M130)