Purple Carbohydrate Fermentation Broth Base with Esculin (BAM M130a)

[所属分类:培养基配方] [发布时间:2021-7-16] [发布人:网站管理员2] [阅读次数:] [返回]

Purple Carbohydrate Fermentation Broth Base
with Esculin
(BAM M130a)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Pancreatic digest of gelatin.........................................................10.0g
NaCl..............................................................................................5.0g
Esculin ..........................................................................................5.0g
Bromcresol Purple ......................................................................0.02g
Carbohydrate solution..............................................................10.0mL
pH 6.8 ± 0.2 at 25°C
Source:  This medium, without esculin, is available as a premixed
powder from BD Diagnostics.
Carbohydrate Solution:
Composition per 100.0mL:
Carbohydrate.................................................................................5.0g
Preparation of Carbohydrate Solution: Add carbohydrate to
distilled/deionized water and bring volume to 100.0mL. Mix thorough-
ly. Filter sterilize.
Preparation of Medium: Add components, except carbohydrate
solution and esculin, to distilled/deionized water and bring volume to
900.0mL. Mix thoroughly. Adjust pH to 7.4 if necessary. Distribute
into tubes containing an inverted Durham tube. Fill each tube with
9.0mL of medium. Add 0.05g esculin to each tube. Autoclave for 15
min at 10 psi pressure–115°C. Aseptically add 1.0mL of sterile carbo-
hydrate solution to each tube.
Use: For the preparation of liquid fermentation media, e.g., for Liste-
ria spp. and Enterococcus spp. Bacteria that can ferment the carbohy-
drate turn the medium yellow.