PYEM Medium (DSMZ Medium 1157)

[所属分类:培养基配方] [发布时间:2021-7-16] [发布人:网站管理员2] [阅读次数:] [返回]

PYEM Medium
(DSMZ Medium 1157)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Peptone ........................................................................................2.0g
Yeast extract .................................................................................2.0g
NH 4 Cl ..........................................................................................0.5g
Riboflavin solution....................................................................5.0mL
Glucose solution........................................................................2.0mL
Magnesium sulfate solution.......................................................1.0mL
Calcium chloride solution..........................................................1.0mL
pH 7.0 ± 0.2 at 25°C
Glucose Solution :
Composition per 10.0mL:
Glucose ........................................................................................5.0g
Preparation of Glucose Solution: Add glucose to distilled/deion-
ized water and bring volume to 10.0mL. Mix thoroughly. Filter steril-
ize.
Riboflavin Solution:
Composition per 10.0ml:
Riboflavin..................................................................................2.0mg
Preparation of Riboflavin Solution:  Add riboflavin to distilled/
deionized water and bring volume to 10.0mL. Mix thoroughly. Filter
sterilize.
Calcium Chloride Solution:
Composition per 10.0mL:
CaC l 2 ·2H 2 O ..................................................................................1.0g
Preparation of Calcium Chloride Solution:  Add CaC l 2 ·2H 2 O
to distilled/deionized water and bring volume to 10.0mL. Mix thor-
oughly. Sparge with N 2 . Autoclave for 15 min at 15 psi pressure–
121°C. Cool to room temperature.
Magnesium Sulfate Solution:
Composition per 10.0mL:
MgSO 4 · 7H 2 O................................................................................2.0g
Preparation of Magnesium Sulfate Solution:  Add MgSO 4 · 7H 2 O
to distilled/deionized water and bring volume to 10.0mL. Mix thor-
oughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to room
temperature.
Preparation of Medium:  Add components, except riboflavin, glu-
cose, magnesium sulfate, and calcium chloride solutions, to distilled/
deionized water and bring volume to 991.0mL. Mix thoroughly. Adjust
pH to 7.0. Autoclave for 15 min at 15 psi pressure–121°C. Cool to
25°C. Aseptically add riboflavin, glucose, magnesium sulfate, and cal-
cium chloride solutions. Mix thoroughly. Aseptically distribute into
culture vessels.
Use:  For the cultivation of Phenylobacterium conjunctum.