PYF Medium

[所属分类:培养基配方] [发布时间:2021-7-29] [发布人:网站管理员2] [阅读次数:] [返回]

PYF Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Yeast extract................................................................................10.0g
Fructose.........................................................................................5.0g
Peptone..........................................................................................5.0g
Pancreatic digest of casein............................................................5.0g
Na 2 HPO 4 .......................................................................................2.0g
L -Cysteine·HCl...........................................................................1.0mL
Tween™ 80................................................................................1.0mL
pH 7.0 ± 0.2 at 25°C
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 7.0.
Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–
121°C.
Use:  For the cultivation of Clostridium acetobutylicum, Megasphaera
cerevisiae, Megasphaera elsdenii, Pectinatus cerevisiiphilus, Pectinatus
frisingensis, Selenomonas lacticifex, Zymophilus paucivorans, and
Zymophilus raffinosivorans.