PYGV Agar

[所属分类:培养基配方] [发布时间:2021-7-29] [发布人:网站管理员2] [阅读次数:] [返回]

PYGV Agar

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................15.0g
Peptone........................................................................................0.25g
Yeast extract................................................................................0.25g
Hutner’s basal salts solution ....................................................20.0mL
Glucose solution ......................................................................10.0mL
Vitamin solution 2X...................................................................5.0mL
pH 7.5 ± 0.2 at 25°C
Hutner’s Basal Salts Solution:
Composition per liter:
MgSO 4 ·7H 2 O..............................................................................29.7g
Nitrilotriacetic acid.....................................................................10.0g
CaCl 2 ·2H 2 O...............................................................................3.335g
FeSO 4 ·7H 2 O.............................................................................99.0mg
(NH 4 ) 6 MoO 7 O 24 ·4H 2 O ............................................................9.25mg
"Metals 44" ..............................................................................50.0mL
"Metals 44":
Composition per 100.0mL:
ZnSO 4 ·7H 2 O.............................................................................1.095g
FeSO 4 ·7H 2 O..................................................................................0.5g
Sodium EDTA.............................................................................0.25g
MnSO 4 ·H2O..............................................................................0.154g
CuSO 4 ·5H 2 O............................................................................39.2mg
Co(NO 3 ) 2 ·6H 2 O .......................................................................24.8mg
Na 2 B 4 O 7 ·10H 2 O.......................................................................17.7mg
Preparation of “Metals 44”: Add sodium EDTA to distilled/de-
ionized water and bring volume to 90.0mL. Mix thoroughly. Add a few
drops of concentrated H 2 SO 4 to retard precipitation of heavy metal
ions. Add remaining components. Mix thoroughly. Bring volume to
100.0mL with distilled/deionized water.
Preparation of Hutner’s Basal Salts Solution:  Add nitrilotria-
cetic acid to 500.0mL of distilled/deionized water. Adjust pH to 6.5
with KOH. Add remaining components. Add distilled/deionized water
to 1.0L. Adjust pH to 6.8.
Glucose Solution:
Composition per 10.0mL:
D -Glucose....................................................................................0.25g
Preparation of Glucose Solution:  Add glucose to distilled/deion-
ized water and bring volume to 10.0mL. Mix thoroughly. Filter steril-
ize.
Vitamin Solution 2 X :
Composition per liter:
Pyridoxine·HCl........................................................................20.0mg
p-Aminobenzoic acid...............................................................10.0mg
Calcium  DL -pantothenate.........................................................10.0mg
Nicotinamide............................................................................10.0mg
Riboflavin................................................................................10.0mg
Thiamine·HCl..........................................................................10.0mg
Biotin.........................................................................................4.0mg
Folic acid ...................................................................................4.0mg
Vitamin B 12 ................................................................................0.2mg
Preparation of Vitamin Solution 2 X :  Add components to dis-
tilled/deionized water and bring volume to 1.0L. Mix thoroughly. Filter
sterilize. Store in the dark at 5°C.
Preparation of Medium: Add components, except glucose solution
and vitamin solution 2X, to distilled/deionized water and bring volume
to 985.0mL. Mix thoroughly. Gently heat and bring to boiling. Adjust
pH to 7.5 with 6N KOH (approximately 6 drops). Autoclave for 20 min
at 15 psi pressure–121°C. Cool to 50°–55°C. Aseptically add 10.0mL
of sterile glucose solution and 5.0mL of sterile vitamin solution 2X.
Mix thoroughly. Pour into sterile Petri dishes or distribute into sterile
tubes.
Use:  For the cultivation of Blastobacter aggregatus, Blastobacter cap-
sulatus, Blastobacter denitrificans, Planctomyces limnophilus, and Gem-
mobacter aquatilis.