Pyrobaculum Medium

[所属分类:培养基配方] [发布时间:2021-7-29] [发布人:网站管理员2] [阅读次数:] [返回]

Pyrobaculum Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Na 2 S 2 O 3 ·5H 2 O..............................................................................2.0g
(NH 4 ) 2 SO 4 .....................................................................................1.3g
Peptone..........................................................................................0.5g
Na 2 S·9H 2 O....................................................................................0.5g
KH 2 PO 4 .......................................................................................0.28g
MgSO 4 ·7H 2 O..............................................................................0.25g
Yeast extract..................................................................................0.2g
CaCl 2 ·2H 2 O.................................................................................0.07g
FeCl 3 ·6H 2 O.................................................................................0.02g
Resazurin ...................................................................................1.0mg
MnCl 2 ·4H 2 O...............................................................................1.8mg
Na 2 B 4 ·10H 2 O.............................................................................4.5mg
ZnSO 4 ·7H 2 O............................................................................0.22mg
CuCl 2 ·2H 2 O .............................................................................0.05mg
Na 2 MoO 4 ·2H 2 O .......................................................................0.03mg
VOSO 4 ·2H 2 O...........................................................................0.03mg
CoSO 4 ......................................................................................0.01mg
Preparation of Medium: Add components, except peptone, yeast
extract, and Na 2 S·9H 2 O, to distilled/deionized water and bring volume
to 1.0L. Mix thoroughly. Bring pH to 6.0 using 8N NaOH. Sparge with
100% N 2 for 30 min. Add peptone, yeast extract, and Na 2 S·9H 2 O.
Bring pH back to 6.0 using 10N H 2 SO 4 . Anaerobically distribute into
sterile tubes or flasks under 100% N 2 . Do not autoclave medium. If not
used immediately, heat the medium to 90°C for 60 min on each of 3
consecutive days.
Use:  For the cultivation and maintenance of Pyrobaculum islandicum.