Pyrococcus Medium

[所属分类:培养基配方] [发布时间:2021-7-30] [发布人:网站管理员2] [阅读次数:] [返回]

Pyrococcus Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Sulfur ..........................................................................................30.0g
NaCl..........................................................................................13.85g
Peptone..........................................................................................5.0g
MgSO 4 ·7H 2 O................................................................................3.5g
MgCl 2 ·6H 2 O ...............................................................................2.75g
Yeast extract..................................................................................1.0g
CaCl 2 ...........................................................................................0.75g
KH 2 PO 4 ........................................................................................0.5g
KCl............................................................................................0.325g
NaBr............................................................................................0.05g
H 3 BO 3 ......................................................................................15.0mg
SrCl 2 ·6H 2 O................................................................................7.5mg
Citric acid...................................................................................5.0mg
(NH 4 ) 2 Ni(SO 4 ) 2 .........................................................................2.0mg
Resazurin ...................................................................................1.0mg
Kl .............................................................................................0.05mg
Trace minerals solution............................................................10.0mL
Na 2 S·9H 2 O solution.................................................................10.0mL
Trace Minerals Solution:
Compostion per liter:
MgSO 4 ·7H 2 O................................................................................3.0g
Nitrilotritracetic acid.....................................................................1.5g
NaCl..............................................................................................1.0g
MnSO 4 ·H 2 O..................................................................................0.5g
CaCl 2 ·2H 2 O..................................................................................0.1g
CoSO 4 (or CoCl 2 ).........................................................................0.1g
FeSO 4 ·7H 2 O .................................................................................0.1g
ZnSO 4 ...........................................................................................0.1g
AIK(SO 4 ) 2 ..................................................................................0.01g
CuSO 4 ·5H 2 O...............................................................................0.01g
H 3 BO 3 .........................................................................................0.01g
Na 2 MoSO 4 2H 2 O.........................................................................0.01g
Preparation of Trace Minerals Solution: Add nitrilotriacetic
acid to 500.0mL of distilled/deionized water. Adjust pH to 6.5 with
KOH. Add remaining components. Add distilled/deionized water to
1.0L. Adjust pH to 7.0.
Na 2 S·9H 2 O Solution:
Composition per 10.0mL:
Na 2 S·9H 2 O....................................................................................0.3g
Preparation of Na 2 S·9H 2 O Solution:  Add Na 2 S·9H 2 O to dis-
tilled/deionized water and bring volume to 10.0mL. Mix thoroughly.
Sparge with 100% N 2 . Autoclave for 15 min at 15 psi pressure–121°C.
Preparation of Medium: Add components, except Na 2 S·9H 2 O so-
lution, to distilled/deionized water and bring volume to 1.0L. Mix thor-
oughly. Adjust pH to 6.5 with H 2 SO 4 . Do not autoclave. Sterilize by
steaming at 100°C for 30 min on 3 consecutive days. Before inocula-
tion, add 10.0mL of sterile Na 2 S·9H 2 O solution. Mix thoroughly.
Use: For the cultivation and maintenance of Pyrococcus furiosus and
Pyrococcus woesei.