Pyrodictium abyssi Medium

[所属分类:培养基配方] [发布时间:2021-7-30] [发布人:网站管理员2] [阅读次数:] [返回]

Pyrodictium abyssi Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Sulfur, powdered.........................................................................30.0g
NaCl..........................................................................................13.85g
MgSO 4 ·7H 2 O................................................................................3.5g
MgCl 2 ·6H 2 O................................................................................2.75g
CaCl 2 ·2H 2 O.................................................................................0.75g
Na 2 S·9H 2 O....................................................................................0.5g
KH 2 PO 4 .........................................................................................0.5g
Yeast extract..................................................................................0.5g
KCl............................................................................................0.325g
NaBr............................................................................................0.05g
H 3 BO 3 .......................................................................................0.015g
(NH 4 ) 2 SO 4 ................................................................................10.0mg
SrCl 2 ·6H 2 O.................................................................................7.5mg
NiCl 2 ·6H 2 O................................................................................2.0mg
Resazurin ...................................................................................1.0mg
Na 2 WO 4 ·2H 2 O...........................................................................0.1mg
KI.............................................................................................0.05mg
Trace elements solution ...........................................................10.0mL
pH 5.5–6.0 at 25°C
Trace Elements Solution:
Composition per liter:
MgSO 4 ·7H 2 O............................................................................... 3.0g
Nitrilotriacetic acid.......................................................................1.5g
NaCl..............................................................................................1.0g
MnSO 4 ·2H 2 O............................................................................... 0.5g
CoSO 4 ·7H 2 O...............................................................................0.18g
ZnSO 4 ·7H 2 O...............................................................................0.18g
CaCl 2 ·2H 2 O...................................................................................0.1g
FeSO 4 ·7H 2 O..................................................................................0.1g
NiCl 2 ·6H 2 O...............................................................................0.025g
KAI(SO 4 ) 2 ·12H 2 O.......................................................................0.02g
CuSO 4 ·5H 2 O...............................................................................0.01g
H 3 BO 3 .........................................................................................0.01g
Na 2 MoO 4 ·2H 2 O ......................................................................... 0.01g
Na 2 SeO 3 ·5H 2 O...........................................................................0.3mg
Preparation of Trace Elements Solution: Add nitrilotriacetic
acid to 500.0mL of distilled/deionized water. Adjust pH to 6.5 with
KOH. Add remaining components. Adjust pH to 7.0. Add distilled/de-
ionized water to 1.0L.
Preparation of Medium: Prepare and dispense medium under 80%
H 2 + 20% CO 2 . Add components, except Na 2 S·9H 2 O, to distilled/de-
ionized water and bring volume to 1.0L. Mix thoroughly. Gently heat
and bring to boiling. Continue boiling for 5 min. Cool to room temper-
ature while sparging with 80% H 2 + 20% CO 2 . Add Na 2 S·9H 2 O. Mix
thoroughly. Bring pH to 5.5 using 10N H 2 SO 4 . Anaerobically distribute
into tubes or flasks making sure to evenly distribute sulfur. Do not au-
toclave. For immediate use, heat the medium in a boiling water bath for
60 min prior to inoculation. For storage of medium, heat medium in a
boiling water bath for 60 min on 3 consecutive days. Store at room tem-
perature.
Use: For the cultivation and maintenance of Pyrodictium abyssi.