Pyruvic Acid Egg Medium

[所属分类:培养基配方] [发布时间:2021-7-30] [发布人:网站管理员2] [阅读次数:] [返回]

Pyruvic Acid Egg Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per 1640.0mL:

KH 2 PO 4 .......................................................................................11.4g
D -Glucose....................................................................................10.0g
Na 2 HPO 4 .......................................................................................6.0g
Pyruvic acid ..................................................................................3.0g
MgSO 4 ·7H 2 O................................................................................0.3g
Malachite Green........................................................................0.125g
Egg, homogenized whole..............................................................1.0L
Penicillin solution....................................................................10.0mL
Source: This medium is available as a prepared medium from Oxoid
Unipath.
Penicillin Solution:
Composition per 10.0mL:
Penicillin G..........................................................................100,000U
Preparation of Penicillin Solution: Add penicillin G to distilled/
deionized water and bring volume to 10.0mL. Mix thoroughly. Filter
sterilize.
Homogenized Whole Egg:
Composition per liter:
Whole eggs ................................................................................18–24
Preparation of Homogenized Whole Egg:  Use fresh eggs, less
than 1 week old. Scrub the shells with soap. Let stand in a soap solution
for 30 min. Rinse in running water. Soak eggs in 70% ethanol for 15
min. Break the eggs into a sterile container. Homogenize by shaking.
Filter through four layers of sterile cheesecloth into a sterile graduated
cylinder. Measure out 1.0L.
Preparation of Medium: Add components, except homogenized
whole egg and penicillin solution, to distilled/deionized water and
bring volume to 630.0mL. Mix thoroughly. Autoclave for 15 min at 15
psi pressure–121°C. Cool to 45°–50°C. Aseptically add homogenized
whole egg and penicillin solution to cooled sterile basal medium. Mix
thoroughly. Aseptically distribute into sterile tubes. Inspissate at 85°–
90°C (moist heat) for 45 min.
Use: For the isolation and cultivation of Mycobacterium species, espe-
cially ones that are drug resistant and difficult to grow.