Quinoline Medium

[所属分类:培养基配方] [发布时间:2021-7-30] [发布人:网站管理员2] [阅读次数:] [返回]

Quinoline Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per 1000.2mL:

K 2 HPO 4 .......................................................................................0.61g
KH 2 PO 4 .......................................................................................0.39g
KCl..............................................................................................0.25g
Yeast extract..................................................................................0.1g
Wolfe’s mineral solution..........................................................10.0mL
Quinoline...................................................................................0.2mL
Wolfe’s Mineral Solution:
Composition per liter :
MgSO 4 ·7H 2 O................................................................................3.0g
Nitrilotriacetic acid.......................................................................1.5g
NaCl..............................................................................................1.0g
MnSO 4 ·2H 2 O................................................................................0.5g
CoCl 2 ·6H 2 O..................................................................................0.1g
ZnSO 4 ·7H 2 O.................................................................................0.1g
CaCl 2 ·2H 2 O ..................................................................................0.1g
FeSO 4 ·7H 2 O..................................................................................0.1g
NiCl 2 ·6H 2 O...............................................................................0.025g
KAl(SO 4 ) 2 ·12H 2 O.......................................................................0.02g
CuSO 4 ·5H 2 O...............................................................................0.01g
H 3 BO 3 .........................................................................................0.01g
Na 2 MoO 4 ·2H 2 O..........................................................................0.01g
Na 2 SeO 3 ·5H 2 O...........................................................................0.3mg
Preparation of Wolfe’s Mineral Solution: Add nitrilotriacetic
acid to 500.0mL of distilled/deionized water. Adjust pH to 6.5 with
KOH. Add remaining components one at a time. Add distilled/deion-
ized water to 1.0L. Adjust pH to 6.8.
Preparation of Medium: Add components, except quinoline, to
distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Autoclave for 15 min at 15 psi pressure–121°C. Cool to room temper-
ature. In a fume hood, aseptically add 0.2mL of quinoline. Mix thor-
oughly. Aseptically distribute into sterile tubes or flasks. Use
polyurethane foam closures to eliminate odors caused by volatilization
of quinoline.
Use: For the cultivation of Rhodococcus species.