R Medium

[所属分类:培养基配方] [发布时间:2021-7-30] [发布人:网站管理员2] [阅读次数:] [返回]

R Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar............................................................................................30.0g
NaHCO 3 ........................................................................................8.0g
K 2 HPO 4 .........................................................................................3.0g
Glucose.........................................................................................2.5g
Glutamine ...................................................................................0.61g
Serine..........................................................................................0.24g
Leucine .......................................................................................0.23g
Lysine..........................................................................................0.23g
Asparagine..................................................................................0.18g
Valine..........................................................................................0.17g
Isoleucine....................................................................................0.17g
Tyrosine ......................................................................................0.14g
Arginine·HCl ............................................................................0.125g
Phenylalanine............................................................................0.125g
Threonine....................................................................................0.12g
Methionine................................................................................0.073g
Glycine......................................................................................0.065g
Histidine·HCl............................................................................0.055g
Proline.......................................................................................0.043g
Tryptophan................................................................................0.035g
L -Cystine...................................................................................0.025g
MgSO 4 ·H 2 O...............................................................................9.9mg
CaCl 2 ·2H 2 O ...............................................................................7.4mg
Adenine sulfate..........................................................................2.1mg
Uracil.........................................................................................1.4mg
Thiamine·HCl............................................................................1.0mg
MnSO 4 ·H 2 O...............................................................................0.9mg
pH 8.0 ± 0.2 at 25°C
Preparation of Medium: Add components, except agar, to dis-
tilled/deionized water and bring volume to 500.0mL. Mix thoroughly.
Filter sterilize. Warm to 45°–50°C. Add agar to distilled/deionized wa-
ter and bring volume to 500.0mL. Mix thoroughly. Autoclave for 15
min at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically combine
both solutions. Mix thoroughly. Pour into sterile Petri dishes or distrib-
ute into sterile tubes.
Use: For the cultivation of Bacillus anthracis, especially for the pro-
duction of toxins.