R2A Agar

[所属分类:培养基配方] [发布时间:2021-7-30] [发布人:网站管理员2] [阅读次数:] [返回]

R2A Agar

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar............................................................................................15.0g
Yeast extract..................................................................................0.5g
Acid hydrolysate of casein............................................................0.5g
Glucose.........................................................................................0.5g
Soluble starch................................................................................0.5g
K 2 HPO 4 .........................................................................................0.3g
Sodium pyruvate...........................................................................0.3g
Pancreatic digest of casein..........................................................0.25g
Peptic digest of animal tissue .....................................................0.25g
MgSO 4 , anhydrous....................................................................0.024g
pH 7.2 ± 0.2 at 25°C
Source:  This medium is available as a premixed powder from BD Di-
agnostic Systems.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat with mix-
ing and bring to boiling. Distribute into tubes or flasks. Autoclave for
15 min at 15 psi pressure–121°C. Do not overheat. Pour into sterile Pe-
tri dishes or leave in tubes.
Use: For use in standard methods for pour plate, spread plate, and
membrane filter analysis to enumerate heterotrophic bacteria from wa-
ters.