R2A Agar, Modified

[所属分类:培养基配方] [发布时间:2021-7-30] [发布人:网站管理员2] [阅读次数:] [返回]

R2A Agar, Modified

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................15.0g
NH 4 Cl ...........................................................................................0.8g
KNO 3 ........................................................................................0.505g
Casamino acids.............................................................................0.5g
Glucose .........................................................................................0.5g
Peptone..........................................................................................0.5g
Sodium pyruvate...........................................................................0.5g
Starch, soluble...............................................................................0.5g
Yeast extract..................................................................................0.5g
K 2 HPO 4 .........................................................................................0.4g
KH 2 PO 4 .......................................................................................0.25g
MgCl 2 ·6H 2 O.............................................................................20.0mg
CaCl 2 ·2H 2 O..............................................................................15.0mg
FeSO 4 ·7H 2 O...............................................................................7.0mg
MnCl 2 ·4H 2 O...............................................................................5.0mg
Na 2 SO 4 .......................................................................................5.0mg
CoCl 2 ·6H 2 O ...............................................................................0.5mg
H 3 BO 3 ........................................................................................0.5mg
NiSO 4 ·6H 2 O...............................................................................0.5mg
ZnCl 2 ..........................................................................................0.5mg
CuCl 2 ·2H 2 O ...............................................................................0.3mg
Na 2 MoO 4 ·2H 2 O ........................................................................10.0μg
pH 7.0 ± 0.2 at 25°C
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 7.0.
Gently heat and bring to boiling. Distribute into tubes or flasks. Auto-
clave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes
or leave in tubes.
Use: For the cultivation of Azoarcus tolulyticus.