R70-2 Agar, Modified with Fructose

[所属分类:培养基配方] [发布时间:2021-7-30] [发布人:网站管理员2] [阅读次数:] [返回]

R70-2 Agar, Modified with Fructose

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Fructose.......................................................................................20.0g
Agar ............................................................................................15.0g
Yeast extract..................................................................................5.0g
Dimethyl glutaric acid ................................................................4.01g
(NH 4 ) 2 SO 4 .....................................................................................3.3g
Trisodium citrate·2H 2 O...............................................................1.18g
KH 2 PO 4 .........................................................................................1.0g
MgSO 4 ·7H 2 O..............................................................................0.25g
Wolfe’s vitamin solution..........................................................10.0mL
100X modified salts.................................................................10.0mL
pH 5.0 ± 0.2 at 25°C
Wolfe’s Vitamin Solution:
Composition per liter:
Pyridoxine·HCl...........................................................................0.01g
Thiamine·HCl ............................................................................5.0mg
Riboflavin..................................................................................5.0mg
Nicotinic acid.............................................................................5.0mg
Calcium pantothenate ................................................................5.0mg
p-Aminobenzoic acid.................................................................5.0mg
Thioctic acid ..............................................................................5.0mg
Biotin.........................................................................................2.0mg
Folic acid ...................................................................................2.0mg
Cyanocobalamin........................................................................0.1mg
Preparation of Wolfe’s Vitamin Solution: Add components to
distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Filter sterilize.
100X Modified Salts:
Composition per liter:
CaCl 2 ·2H 2 O ................................................................................1.47g
FeCl 3 ·6H 2 O.................................................................................0.27g
ZnSO 4 ·7H 2 O.............................................................................0.144g
MnSO 4 ·H 2 O..............................................................................0.085g
CoCl 2 ·6H 2 O..............................................................................0.024g
NiCl 2 ·6H 2 O...............................................................................0.024g
Na 2 MoO 4 ·2H 2 O........................................................................0.024g
CuSO 4 ·5H 2 O.............................................................................0.016g
HCl, concentrated......................................................................4.1mL
Preparation of 100X Modified Salts: Add components to dis-
tilled/deionized water and bring volume to 1.0L. Mix thoroughly. Filter
sterilize.
Preparation of Medium: Add components, except Wolfe’s vitamin
solution and 100X modified salts, to distilled/deionized water and
bring volume to 980.0mL. Mix thoroughly. Adjust pH to 5.0. Gently
heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–
121°C. Cool to 45°–50°C. Aseptically add sterile Wolfe’s vitamin so-
lution and 100X modified salts. Mix thoroughly. Pour into sterile Petri
dishes or distribute into sterile tubes.
Use: For the cultivation of Acetobacter xylinum.