Rabbit Blood Agar

[所属分类:培养基配方] [发布时间:2021-8-2] [发布人:网站管理员2] [阅读次数:] [返回]

Rabbit Blood Agar

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per 1250.0mL:

Pancreatic digest of casein..........................................................16.0g
Agar ............................................................................................13.5g
Brain heart, solids from infusion ..................................................8.0g
Peptic digest of animal tissue........................................................5.0g
NaCl..............................................................................................5.0g
Glucose .........................................................................................2.0g
Na 2 HPO 4 .......................................................................................2.5g
Rabbit blood, defibrinated .....................................................250.0mL
pH 7.4 ± 0.2 at 25°C
Preparation of Medium: Add components, except rabbit blood, to
distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Autoclave for 15 min at 15 psi–121°C. Aseptically add sterile rabbit
blood. Pour into sterile Petri dishes or aseptically distribute into sterile
tubes or flasks while shaking.
Use: For the cultivation and maintenance of Corynebacterium diph-
theriae, Haemophilus ducreyi, and Actinobacillus lignieresii.