RajHans Medium (HiCrome™ Salmonella Medium, Modified)

[所属分类:培养基配方] [发布时间:2021-8-2] [发布人:网站管理员2] [阅读次数:] [返回]

RajHans Medium
(HiCrome™ Salmonella Medium, Modified)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar ...........................................................................................12.0 g
Casein enzymic hydrolysate ........................................................8.0 g
Yeast extract.................................................................................5.0 g
NaCl.............................................................................................5.0 g
Chromogenic mixture................................................................4.32 g
Peptic digest of animal tissue.......................................................4.0 g
Lactose.........................................................................................3.0 g
Sodium deoxycholate...................................................................1.0 g
Neutral Red................................................................................0.02 g
pH 7.3 ± 0.2 at 25°C
Source: This medium is available as a premixed powder from Hi-
Media.
Preparation of Medium:  Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat while
stirring and bring to boiling. Mix to completely dissolve components.
Do not autoclave. Cool to 50°C. Mix thoroughly. Pour into sterile Petri
dishes.
Use: A selective chromgenic medium used for the isolation and differ-
entiation of Salmonella species from the members of Enterobacteri-
aceae, especially Proteus species.