Raka-Ray Agar

[所属分类:培养基配方] [发布时间:2021-8-2] [发布人:网站管理员2] [阅读次数:] [返回]

Raka-Ray Agar

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Pancreatic digest of casein..........................................................20.0g
Agar............................................................................................17.0g
Maltose .......................................................................................10.0g
Fructose.........................................................................................5.0g
Glucose.........................................................................................5.0g
Yeast extract..................................................................................5.0g
2-Phenylethanol............................................................................3.0g
Potassium aspartate.......................................................................2.5g
Potassium glutamate.....................................................................2.5g
Betaine·HCL.................................................................................2.0g
Diammonium hydrogen citrate.....................................................2.0g
MgSO 4 ·7H 2 O................................................................................2.0g
KH 2 PO 4 .........................................................................................2.0g
Liver concentrate..........................................................................1.0g
MnSO 4 ·H 2 O................................................................................0.66g
N-Acetylglucosamine...................................................................0.5g
Cycloheximide...........................................................................7.0mg
Sorbitan monooleate................................................................10.0mL
pH 5.4 ± 0.2 at 25°C
Source:  This medium is available as a premixed powder from Oxoid
Unipath.
Caution:  Cycloheximide is toxic. Avoid skin contact or aerosol for-
mation and inhalation.
Preparation of Medium: Add components, except phenylethanol,
to distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pres-
sure–121°C. Cool to 45°–50°C. Aseptically add 3.0g of 2-phenyletha-
nol. Mix thoroughly. Pour into sterile Petri dishes or distribute into
sterile tubes.
Use: For the isolation of lactic acid bacteria in beer and brewing pro-
cesses.