Rambach ® Agar

[所属分类:培养基配方] [发布时间:2021-8-2] [发布人:网站管理员2] [阅读次数:] [返回]

Rambach ® Agar

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................15.0g
Polypropylene glycol..................................................................10.5g
Peptone..........................................................................................8.0g
NaCl..............................................................................................5.0g
Chromogenic mix .........................................................................1.5g
Na-desoxycholate..........................................................................1.0g
pH 7.4 ± 0.2 at 25°C
Source:  Rambach Agar is available from CHROMagar Microbiology
and Merck.
Preparation of Medium:  Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Heat in a boiling wa-
ter bath or in a current of steam, while shaking from time to time. The
medium is totally suspended, if no visual particles stick to the glass
wall. The medium should not be heat treated further. Complete disso-
lution with shaking in 5-min sequences is approximately 35–40 min-
utes. Do not autoclave. Do not overheat. Cool as fast as possible to
45°–50°C while gently shaking from time to time. Pour into sterile Pe-
tri dishes. To prevent any precipitate or clotting of the chromogenic
mix in the plates, place Petri dishes during pouring procedure on a cool
(max. 25°C) surface. The plates are opaque and pink.
Use:  For the detection of enteric bacteria, including coliforms and Sal-
monella spp. Sodium desoxycholate inhibits the accompanying Gram-
positive flora. This medium enables Salmonella spp. to be differenti-
ated unambiguously from other bacteria. Salmonella spp. form a char-
acteristic red color. In order to differentiate coliforms from Salmonel-
lae, the medium contains a chromogene indicating the presence of β-
galactosidase splitting, a characteristic of coliforms. Coliform micro-
organisms grow as blue-green or blue-violet colonies. Other Enter-
obacteriaceae and Gram-negative bacteria, such as Proteus,
Pseudomonas, Shigella, S. typhi, and S. parathyphi A, grow as color-
less-yellow colonies.