Rapid HiColiform Agar

[所属分类:培养基配方] [发布时间:2021-8-2] [发布人:网站管理员2] [阅读次数:] [返回]

Rapid HiColiform Agar

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................15.0g
Peptone, special ............................................................................5.0g
NaCl..............................................................................................5.0g
K 2 HPO 4 .........................................................................................2.7g
KH 2 PO 4 .........................................................................................2.0g
Sorbitol..........................................................................................1.0g
Sodium lauryl sulfate....................................................................0.1g
1-Isopropyl- ß- D -1-thiogalactopyranoside....................................0.1g
Chromogenic mixture.................................................................0.08g
Fluorogenic mixture....................................................................0.05g
pH 7.2 ± 0.2 at 25°C
Source: This medium is available as a premixed powder from Hi-
Media.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15
psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.
Use:  For the detection and confirmation of Escherichia coli and total
coliforms on the basis of enzyme substrate reaction from water sam-
ples, using a combination of chromogenic and fluorogenic substrates.