Rappaport Broth, Modified (Rap Broth, Modified)

[所属分类:培养基配方] [发布时间:2021-8-2] [发布人:网站管理员2] [阅读次数:] [返回]

Rappaport Broth, Modified
(Rap Broth, Modified)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per 250.2mL:

Solution A..............................................................................155.0mL
Solution C................................................................................53.0mL
Solution B................................................................................40.0mL
Solution D..................................................................................1.6mL
Solution E ..................................................................................0.6mL
Solution A:
Composition per liter:
Pancreatic digest of casein..........................................................10.0g
Preparation of Solution A: Add pancreatic digest of casein to dis-
tilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Solution B:
Composition per liter:
Na 2 HPO 4 .......................................................................................9.5g
Preparation of Solution B: Add Na 2 HPO 4 to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly.
Solution C:
Composition per 100.0mL:
MgCl 2 ·6H 2 O ...............................................................................40.0g
Preparation of Solution C: Add MgCl 2 ·6H 2 O to distilled/deion-
ized water and bring volume to 100.0mL. Mix thoroughly. Autoclave
for 15 min at 15 psi pressure–121°C. Cool to 25°C.
Solution D:
Composition per 100.0mL:
Malachite Green............................................................................0.2g
Preparation of Solution D: Add Malachite Green to sterile dis-
tilled/deionized water and bring volume to 100.0mL. Mix thoroughly.
Do not sterilize.
Solution E:
Composition per 10.0mL:
Carbenicillin ...............................................................................0.01g
Preparation of Solution E:  Add carbenicillin to distilled/deionized
water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize.
Preparation of Medium: Combine 155.0mL of solution A and
40.0mL of solution B. Mix thoroughly. Autoclave for 15 min at 15 psi
pressure–121°C. Cool to 45°–50°C. Aseptically add 53.0mL of sterile
solution C, 1.6mL of solution D, and 0.6mL of sterile solution E. Mix
thoroughly. Aseptically distribute into sterile tubes or flasks.
Use:  For the isolation and cultivation of Yersinia enterocolitica from
foods.