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Rappaport-Vassiliadis Medium Semisolid, Modified with Novobiocin (MSRV)
[所属分类:培养基配方] [发布时间:2021-8-2] [发布人:网站管理员2] [阅读次数:] [返回]
Rappaport-Vassiliadis Medium Semisolid, Modified
with Novobiocin
(MSRV)
NaCl............................................................................................7.34g
Enzymatic digest of casein .........................................................4.59g
Casein acid hydrolysate..............................................................4.59g
Agar ..............................................................................................2.7g
KH 2 PO 4 .......................................................................................1.4 g
Malachite Green oxalate...........................................................0.037g
Novobiocin solution.................................................................. 1.0mL
pH 5.6 ± 0.2 at 25°C
Novobiocin Solution:
Composition per 10.0mL:
Novobiocin....................................................................................0.2g
Preparation of Novobiocin Solution: Add novobiocin to dis-
tilled/deionized water and bring volume to 10.0mL. Mix thoroughly.
Filter sterilize.
Preparation of Medium: Add components, except novobiocin so-
lutiont, to distilled/deionized water and bring volume to 990.0mL. Mix
thoroughly. Gently heat and bring to boiling. Distribute into tubes or
flasks. Do not autoclave. Do not overheat. Cool to 50°C. Aseptically
add 1.0mL novobiocin solution. Pour into sterile Petri dishes or leave
in tubes.
Use: For the rapid and sensitive isolation of motile Salmonella spp.
from food products following pre-enrichment or selective enrichment.
The semisolid medium allows motility to be detected as halos of
growth around the original point of inoculation. Recommended by the
European Chocolate Manufacturer’s Association. For the isolation of f
Salmonella spp. (other than S. typhi and S. partyphi type A) from stool
specimens with high sensitivity and specificity.
with Novobiocin
(MSRV)
山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com
Composition per liter:
MgCl 2 ........................................................................................10.93gNaCl............................................................................................7.34g
Enzymatic digest of casein .........................................................4.59g
Casein acid hydrolysate..............................................................4.59g
Agar ..............................................................................................2.7g
KH 2 PO 4 .......................................................................................1.4 g
Malachite Green oxalate...........................................................0.037g
Novobiocin solution.................................................................. 1.0mL
pH 5.6 ± 0.2 at 25°C
Novobiocin Solution:
Composition per 10.0mL:
Novobiocin....................................................................................0.2g
Preparation of Novobiocin Solution: Add novobiocin to dis-
tilled/deionized water and bring volume to 10.0mL. Mix thoroughly.
Filter sterilize.
Preparation of Medium: Add components, except novobiocin so-
lutiont, to distilled/deionized water and bring volume to 990.0mL. Mix
thoroughly. Gently heat and bring to boiling. Distribute into tubes or
flasks. Do not autoclave. Do not overheat. Cool to 50°C. Aseptically
add 1.0mL novobiocin solution. Pour into sterile Petri dishes or leave
in tubes.
Use: For the rapid and sensitive isolation of motile Salmonella spp.
from food products following pre-enrichment or selective enrichment.
The semisolid medium allows motility to be detected as halos of
growth around the original point of inoculation. Recommended by the
European Chocolate Manufacturer’s Association. For the isolation of f
Salmonella spp. (other than S. typhi and S. partyphi type A) from stool
specimens with high sensitivity and specificity.
