Raymond’s Medium

[所属分类:培养基配方] [发布时间:2021-8-3] [发布人:网站管理员2] [阅读次数:] [返回]

Raymond’s Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Na 2 HPO 4 ......................................................................................3.0g
NaCl..............................................................................................3.0g
NH 4 NO 3 ........................................................................................2.0g
KH 2 PO 4 ........................................................................................2.0g
MgSO 4 ..........................................................................................0.2g
Na 2 CO 3 ........................................................................................0.1g
MnSO 4 ........................................................................................0.02g
CaCl 2 ..........................................................................................0.01g
FeSO 4 ..........................................................................................0.01g
n-Hexadecane ..........................................................................10.0mL
pH 6.8–7.0 at 25°C
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes
or flasks. Autoclave for 20 min at 15 psi pressure–121°C.
Use:  For the cultivation of Rhodococcus erythropolis, Rhodococcus
luteus, Rhodococcus maris, and other bacteria that can utilize n-hexa-
decane as a carbon source.