RCM Medium, Modified

[所属分类:培养基配方] [发布时间:2021-8-3] [发布人:网站管理员2] [阅读次数:] [返回]

RCM Medium, Modified

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Casamino acids...........................................................................15.0g
Tryptose......................................................................................10.0g
Beef extract.................................................................................10.0g
Glucose.........................................................................................5.0g
NaCl..............................................................................................5.0g
Yeast extract..................................................................................3.0g
Sodium acetate..............................................................................3.0g
Soluble starch................................................................................1.0g
L -Cysteine·HCl·H 2 O.....................................................................0.5g
Agar..............................................................................................0.5g
pH 6.8 ± 0.2 at 25°C
Source:  Reinforced clostridial medium without casamino acids is
available as a premixed powder from BD Diagnostic Systems and Ox-
oid Unipath.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 10
psi pressure–115°C. Pour into sterile Petri dishes or leave in tubes.
Use:  For the cultivation of Clostridium acetireducens and Clostridium
aminophilum.