R-CW Medium (LMG Medium 265)

[所属分类:培养基配方] [发布时间:2021-8-3] [发布人:网站管理员2] [阅读次数:] [返回]

R-CW Medium
(LMG Medium 265)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Trypticase™..................................................................................5.0g
Tryptone........................................................................................5.0g
Yeast extract..................................................................................5.0g
KH 2 PO 4 .........................................................................................5.0g
Na-acetate .....................................................................................5.0g
(NH 4 ) 2 citrate.................................................................................2.0g
MgSO 4 ·7H 2 O................................................................................0.5g
MnSO 4 ·5H 2 O................................................................................0.2g
Cheese whey.................................................................................1.0L
Tween™ 80................................................................................1.0mL
pH 5.5 ± 0.2 at 25°C
Preparation of Medium:  Add components to 1.0L cheese whey.
Mix thoroughly. Distribute into tubes or flasks. Autoclave for 15 min
at 15 psi pressure–121°C.
Use: For cultivation and maintenance of Lactobacillus kefirgranum
and Lactobacillus parakefiri.