Reactivation with Liquid Medium 246 (DSMZ Medium 246a)

[所属分类:培养基配方] [发布时间:2021-8-3] [发布人:网站管理员2] [阅读次数:] [返回]

Reactivation with Liquid Medium 246
(DSMZ Medium 246a)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Peptone........................................................................................10.0g
Yeast extract................................................................................10.0g
Artificial seawater, filtered ....................................................750.0mL
pH 7.3 ± 0.2 at 25°C
Artificial Seawater:
Composition per liter:
NaCl..........................................................................................28.13g
MgCl 2 ·6H 2 O..................................................................................4.8g
MgSO 4 ·7H 2 O................................................................................3.5g
CaCl 2 ·2H 2 O...................................................................................1.6g
KCl..............................................................................................0.77g
NaHCO 3 ......................................................................................0.11g
Preparation of Artificial Seawater: Add components to distilled/
deionized water and bring volume to 1.0L. Mix thoroughly. Filter ster-
ilize.
Preparation of Medium: Add peptone and yeast extract to 250.0mL
tap water. Mix thoroughly. Adjust pH to 7.8. Boil for 5 min. Filter and
readjust the pH to 7.3. Distribute into tubes or flasks. Autoclave for 15
min at 15 psi pressure–121°C. Cool to 25°C. Add 750.0mL filter ster-
ilized seawater. (Note: Natural seawater is stored in the dark for at least
3 weeks to age. If natural seawater is not available use artificial seawa-
ter.) Mix thoroughly.
Use: For the rehydration and cultivation of Photorhabdus lumine-
scens=Xenorhabdus luminescens and marine bacteria from lyophilized
ampules of stock cultures.