Regan-Lowe Charcoal Agar (Regan-Lowe Medium)

[所属分类:培养基配方] [发布时间:2021-8-3] [发布人:网站管理员2] [阅读次数:] [返回]

Regan-Lowe Charcoal Agar
(Regan-Lowe Medium)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar............................................................................................12.0g
Beef extract.................................................................................10.0g
Pancreatic digest of gelatin.........................................................10.0g
Soluble starch..............................................................................10.0g
NaCl..............................................................................................5.0g
Charcoal........................................................................................4.0g
Niacin..........................................................................................0.01g
Horse blood, defibrinated ......................................................100.0mL
Cephalexin solution.................................................................10.0mL
pH 7.4 ± 0.2 at 25°C
Source:  This medium is available as a premixed powder from BD Di-
agnostic Systems.
Cephalexin Solution:
Composition per 10.0mL:
Cephalexin..................................................................................0.04g
Preparation of Cephalexin Solution: Add cephalexin to dis-
tilled/deionized water and bring volume to 10.0mL. Mix thoroughly.
Filter sterilize.
Preparation of Medium: Add components, except horse blood and
cephalexin solution, to distilled/deionized water and bring volume to
890.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave
for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically
add sterile horse blood and sterile cephalexin solution. Mix thoroughly.
Pour into sterile Petri dishes or distribute into sterile tubes. Swirl me-
dium while dispensing to keep charcoal in suspension.
Use: For the selective isolation and cultivation of Bordetella pertussis
and Bordetella parapertussis from clinical specimens.