Reinforced AE Medium (RAE Medium) (LMG Medium 239)

[所属分类:培养基配方] [发布时间:2021-8-3] [发布人:网站管理员2] [阅读次数:] [返回]

Reinforced AE Medium
(RAE Medium)
(LMG Medium 239)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Base medium..........................................................................500.0mL
Growth medium.....................................................................500.0mL
pH 5.0 ± 0.2 at 25°C
Base Medium:
Composition per liter:
Agar ............................................................................................10.0g
Preparation of Base Medium: Add agar to distilled/deionized wa-
ter and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to
boiling. Autoclave for 15 min at 15 psi pressure–121°C. Pour as a base
layer into sterile Petri dishes.
Growth Medium:
Composition per liter:
Glucose .......................................................................................40.0g
Agar ............................................................................................20.0g
Yeast extract................................................................................10.0g
Peptone........................................................................................10.0g
Na 2 HPO 4 ·2H 2 O...........................................................................3.38g
Citric acid·2H 2 O............................................................................1.5g
Ethanol.....................................................................................20.0mL
Acetic acid ...............................................................................10.0mL
Preparation of Growth Medium: Add components, except etha-
nol and acetic acid, to distilled/deionized water and bring volume to
970.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave
for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically
add 20.0mL filter sterilized ethanol and 10.0mL filter sterilized acetic
acid. Mix thoroughly.
Preparation of Growth Medium: This medium is used as a dou-
ble layer. Pour as a layer of base medium into sterile Petri dishes. Al-
low to solidify. Pour a thin layer of growth medium over the solid base
medium. Allow to solidify.
Use: For the isolation and cultivation of Gluconacetobacter spp. and
Acetobacter pomorum.