Rhodobium Medium (DSMZ Medium 745)

[所属分类:培养基配方] [发布时间:2021-8-4] [发布人:网站管理员2] [阅读次数:] [返回]

Rhodobium Medium
(DSMZ Medium 745)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

NaCl............................................................................................50.2g
Na- DL -malate................................................................................3.6g
Yeast extract..................................................................................1.0g
KH 2 PO 4 .........................................................................................1.0g
(NH 4 ) 2 SO 4 .....................................................................................1.0g
MgCl 2 ·6H 2 O..................................................................................0.2g
CaCl 2 ·2H 2 O.................................................................................0.05g
Na 2 S·9H 2 O solution.................................................................10.0mL
Trace elements solution SL-8 ....................................................1.0mL
pH 6.8 ± 0.2 at 25°C
Trace Elements Solution SL-8:
Composition per liter:
Na 2 -EDTA.....................................................................................5.2g
FeCl 2 ·4H 2 O...................................................................................1.5g
CoCl 2 ·6H 2 O ...........................................................................190.0mg
MnCl 2 ·4H 2 O...........................................................................100.0mg
ZnCl 2 ........................................................................................70.0mg
H 3 BO 3 ......................................................................................62.0mg
Na 2 MoSO 4 ·2H 2 O.....................................................................36.0mg
NiCl 2 ·6H 2 O..............................................................................24.0mg
CuCl 2 ·2H 2 O .............................................................................17.0mg
Preparation of Trace Elements Solution SL-8: Add compo-
nents to distilled/deionized water and bring volume to 1.0L. Mix thor-
oughly. Sparge with 100% N 2 .
Na 2 S·9H 2 O Solution :
Composition per 10.0mL:
Na 2 S·9H 2 O....................................................................................0.5g
Preparation of Na 2 S·9H 2 O Solution: Add Na 2 S·9H 2 O to dis-
tilled/deionized water and bring volume to 10.0mL. Mix thoroughly.
Adjust pH to 6.8. Autoclave under 100% N 2 for 15 min at 15 psi pres-
sure–121°C. Cool to room temperature.
Preparation of Medium: Add components, except Na 2 S·9H 2 O so-
lution, to distilled/deionized water and bring volume to 1.0L. Mix thor-
oughly. Adjust to pH 6.8. Gently heat and bring to boiling. Cool to
room temperature while sparging with 100% N 2 . Autoclave for 15 min
at 15 psi pressure–121°C. Cool to room temperature. Add 10.0mL ster-
ile Na 2 S·9H 2 O solution. Mix thoroughly. Aseptically and anaerobical-
ly under 100% N 2  distribute into sterile screw-cap tubes or flasks.
Use: For the cultivation of Rhodobium orientis (Rhodovulum orientis).