Rhodovulum visakhum Medium (DSMZ Medium 1128)

[所属分类:培养基配方] [发布时间:2021-8-5] [发布人:网站管理员2] [阅读次数:] [返回]

Rhodovulum visakhum Medium
(DSMZ Medium 1128)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

NaCl ...........................................................................................20.0g
MgCl 2 · 6H 2 O..................................................................................1.0g
NH 4 Cl ..........................................................................................0.6g
KH 2 PO 4 .........................................................................................0.5g
Sorbitol .........................................................................................3.0g
Sodium pyruvate ..........................................................................3.0g
Yeast extract .................................................................................0.4g
CaC l 2 ·2H 2 O.................................................................................0.15g
Trace elements solution SL-8 ....................................................1.0mL
Vitamin solution.........................................................................1.0mL
pH 6.5 ± 0.2 at 25°C
Trace Elements Solution SL-8:
Composition per liter:
Disodium EDTA ...........................................................................5.2g
FeCl 2 ·4H 2 O...................................................................................1.5g
CoCl 2 ·6H 2 O................................................................................0.19g
MnCl 2 ·4H 2 O .................................................................................0.1g
ZnCl 2 ...........................................................................................0.07g
H 3 BO 3 .........................................................................................0.06g
NaMoO 4 ·2H 2 O............................................................................0.04g
CuCl 2 ·2H 2 O................................................................................0.02g
NiCl 2 ·6H 2 0..................................................................................0.02g
Preparation of Trace Elements Solution SL-8: Add compo-
nents to distilled/deionized water and bring volume to 1.0L. Mix thor-
oughly. Filter sterilize.
Vitamin Solution:
Composition per 10.0mL:
Vitamin B 12 ................................................................................0.2mg
Preparation of Vitamin Solution:  Add components to distilled/de-
ionized water and bring volume to 10.0mL. Mix thoroughly. Filter ster-
ilize.
Preparation of Medium: Add components, except trace elements
SL-8 and vitamin solutions, to distilled/deionized water and bring vol-
ume to 1.0L. Mix thoroughly. Adjust pH to 6.5. Distribute into tubes or
flasks. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi
pressure–121°C. Cool to room temperature. Aseptically add trace ele-
ments SL-8 and vitamin solutions. Mix thoroughly. Adjust pH to 8.3.
Aseptically distribute into culture vessels.
Use: For the cultivation of Rhodovulum visakhum.