Rogosa SL Agar (Rogosa Selective Lactobacillus Agar)

[所属分类:培养基配方] [发布时间:2021-8-6] [发布人:网站管理员2] [阅读次数:] [返回]

Rogosa SL Agar
(Rogosa Selective Lactobacillus Agar)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................15.0g
Sodium acetate............................................................................15.0g
Glucose .......................................................................................10.0g
Pancreatic digest of casein..........................................................10.0g
K 2 HPO 4 .........................................................................................6.0g
Yeast extract..................................................................................5.0g
Arabinose......................................................................................5.0g
Sucrose..........................................................................................5.0g
Ammonium citrate........................................................................2.0g
Sorbitan monooleate.....................................................................1.0g
MgSO 4 ·7H 2 O..............................................................................0.57g
MnSO 4 ·7H 2 O..............................................................................0.12g
FeSO 4 ·H 2 O..................................................................................0.03g
Acetic acid, glacial...................................................................1.32mL
pH 5.4 ± 0.2 at 25°C
Source:  This medium is available as a premixed powder from BD Di-
agnostic Systems.
Preparation of Medium: Add components, except glacial acetic
acid, to distilled/deionized water and bring volume to 998.7mL. Mix
thoroughly. Gently heat and bring to boiling. Add glacial acetic acid.
Mix thoroughly. Gently heat while stirring and bring to 90°–100°C for
2–3 min. Do not autoclave. Pour into sterile Petri dishes or distribute
into sterile tubes.
Use: For the isolation, cultivation, and enumeration of lactobacilli,
especially from feces, saliva, vaginal specimens, and dairy products.