Rouf's Medium (DSMZ Medium 1019)

[所属分类:培养基配方] [发布时间:2021-8-6] [发布人:网站管理员2] [阅读次数:] [返回]

Rouf's Medium
(DSMZ Medium 1019)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Yeast extract .................................................................................5.0g
Peptone ........................................................................................5.0g
MgSO 4 ·7H 2 O................................................................................0.2g
Fe(NH3)citrate ...........................................................................0.15g
CaC l 2 ·2H 2 O ................................................................................0.05g
MnSO 4 ·H 2 O................................................................................0.05g
FeCl 3 ·6H 2 O.................................................................................0.01g
Vitamin solution.......................................................................10.0mL
Trace elements solution ............................................................1.0mL
pH 7.1 ± 0.2 at 25°C
Vitamin Solution:
Composition per liter:
Pyridoxine-HCl........................................................................10.0mg
Thiamine-HCl·2H 2 O..................................................................5.0mg
Riboflavin..................................................................................5.0mg
Nicotinic acid.............................................................................5.0mg
D -Ca-pantothenate .....................................................................5.0mg
p-Aminobenzoic acid.................................................................5.0mg
Lipoic acid.................................................................................5.0mg
Biotin.........................................................................................2.0mg
Folic acid ...................................................................................2.0mg
Vitamin B 12 ................................................................................0.1mg
Preparation of Vitamin Solution:  Add components to distilled/
deionized water and bring volume to 1.0L. Mix thoroughly. Filter ster-
ilize.
Trace Elements Solution:
Composition per liter:
Nitrilotriacetic acid.....................................................................12.8g
CoCl 2 ·6H 2 O................................................................................0.17g
CaCl 2 ·2H 2 O ..................................................................................0.1g
FeSO 4 ·7H 2 O..................................................................................0.1g
MnCl 2 ·4H 2 O..................................................................................0.1g
NaCl..............................................................................................0.1g
Na 2 MoO 4 ·2H 2 O ............................................................................0.1g
Preparation of Trace Elements Solution:  Add nitrilotriacetic
acid to 500.0mL of distilled/deionized water. Dissolve by adjusting pH
to 6.5 with KOH. Add remaining components. Add distilled/deionized
water to 1.0L. Mix thoroughly. Adjust pH to 7.0.
Preparation of Medium: Add components, except trace elements
and vitamin solutions, to distilled/deionized water and bring volume to
989.0mL. Mix thoroughly. Adjust pH to 7.1. Distribute into tubes or
flasks. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi
pressure–121°C. Cool to room temperature. Aseptically add trace ele-
ments and vitamin solutions. Mix thoroughly. Adjust pH to 7.1. Asep-
tically distribute into culture vessels.
Use: For the cultivation of Phenylobacterium lituiforme.