RPMI 1640 Medium with L -Glutamine

[所属分类:培养基配方] [发布时间:2021-8-6] [发布人:网站管理员2] [阅读次数:] [返回]

RPMI 1640 Medium with  L -Glutamine

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

NaCl..............................................................................................6.0g
NaHCO 3 ........................................................................................2.0g
D- Glucose......................................................................................2.0g
Na 2 HPO 4 ·7H 2 O.............................................................................1.5g
KCl................................................................................................0.4g
L- Glutamine...................................................................................0.3g
L- Arginine .....................................................................................0.2g
Ca(NO 3 ) 2 · 4 H 2 O.............................................................................0.1g
MgSO 4 ·7H 2 O................................................................................0.1g
L- Asparagine ...............................................................................0.05g
L- Cystine .....................................................................................0.05g
L- Isoleucine, allo free..................................................................0.05g
L- Leucine, methionine free .........................................................0.05g
L- Lysine·HCl...............................................................................0.04g
i-Inositol....................................................................................0.035g
L- Serine .......................................................................................0.03g
L- Aspartic acid ............................................................................0.02g
L- Glutamic acid...........................................................................0.02g
L- Hydroxyproline........................................................................0.02g
L- Proline, hydroxy- L- proline free................................................0.02g
L- Threonine, allo free..................................................................0.02g
L- Tyrosine....................................................................................0.02g
L- Valine .......................................................................................0.02g
L- Histidine, free base.................................................................0.015g
L- Methionine.............................................................................0.015g
L- Phenylalanine.........................................................................0.015g
Glycine........................................................................................0.01g
L- Tryptophan..............................................................................5.0mg
Phenol Red.................................................................................5.0mg
Choline chloride.........................................................................3.0mg
Glutathione, reduced..................................................................1.0mg
p-Aminobenzoic acid.................................................................1.0mg
Folic acid....................................................................................1.0mg
Nicotinamide..............................................................................1.0mg
Pyridoxine·HCl..........................................................................1.0mg
Thiamine·HCl ............................................................................1.0mg
D- Calcium pantothenate...........................................................0.25mg
Biotin .........................................................................................0.2mg
Riboflavin ..................................................................................0.2mg
Vitamin B 12 .................................................................................5.0μg
pH 7.3 ± 0.2 at 25°C
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Adjust pH to 7.3 with 1N HCl or 1N
NaOH. Filter sterilize. Aseptically distribute into sterile tubes or flasks.
Use: For the cultivation of mammalian cells in tissue culture. Culture
media for human immunodeficiency viruses.