SC Broth

[所属分类:培养基配方] [发布时间:2021-8-11] [发布人:网站管理员2] [阅读次数:] [返回]

SC Broth

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Papaic digest of soybean meal......................................................8.0g
KH 2 PO 4 .........................................................................................1.5g
K 2 HPO 4 .........................................................................................0.5g
MgSO 4 ·7H 2 O................................................................................0.2g
Hemin solution.........................................................................15.0mL
Bovine serum albumin, fraction V solution.............................10.0mL
L -Cysteine·H 2 O solution..........................................................10.0mL
Glucose solution........................................................................1.0mL
pH 6.6 ± 0.2 at 25°C
Hemin Solution:
Composition per 100.0mL:
Hemin ...........................................................................................0.1g
NaOH (0.05N solution)..........................................................100.0mL
Preparation of Hemin Solution: Add hemin to NaOH solution.
Mix thoroughly.
Bovine Serum Albumin, Fraction V Solution:
Composition per 10.0mL:
Bovine serum albumin, fraction V................................................2.0g
Preparation of Bovine Serum Albumin, Fraction V Solu-
tion: Add bovine serum albumin to distilled/deionized water and bring
volume to 10.0mL. Mix thoroughly. Filter sterilize.
L -Cysteine·H 2 O Solution:
Composition per 10.0mL:
L -Cysteine·H 2 O.............................................................................1.0g
Preparation of  L -Cysteine·H 2 O Solution: Add  L -cysteine·H 2 O
to distilled/deionized water and bring volume to 10.0mL. Mix thor-
oughly. Filter sterilize.
Glucose Solution:
Composition per 10.0mL:
D- Glucose......................................................................................5.0g
Preparation of Glucose Solution: Add glucose to distilled/deion-
ized water and bring volume to 10.0mL. Mix thoroughly. Filter steril-
ize.
Preparation of Medium: Add components—except bovine serum
albumin solution,  L -cysteine·H 2 O solution, and glucose solution—to
distilled/deionized water and bring volume to 979.0mL. Mix thorough-
ly. Adjust pH to 6.6 with NaOH. Gently heat and bring to boiling. Au-
toclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C.
Aseptically add 10.0mL of sterile bovine serum albumin solution,
10.0mL of sterile  L -cysteine·H 2 O solution, and 1.0mL of sterile glu-
cose solution. Mix thoroughly. Aseptically distribute into sterile tubes
or flasks.
Use:  For the cultivation of Clavibacter xyli.