Schaedler Broth (Schaedler Anaerobic Broth)

[所属分类:培养基配方] [发布时间:2021-8-12] [发布人:网站管理员2] [阅读次数:] [返回]

Schaedler Broth
(Schaedler Anaerobic Broth)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Pancreatic digest of casein............................................................8.2g
Glucose.........................................................................................5.8g
Yeast extract..................................................................................5.0g
Tris(hydroxymethyl)aminomethane buffer...................................3.0g
Peptic digest of animal tissue .......................................................2.5g
NaCl..............................................................................................1.7g
Papaic digest of soybean meal......................................................1.0g
K 2 HPO 4 .........................................................................................0.8g
L- Cystine.......................................................................................0.4g
Hemin .........................................................................................0.01g
pH 7.6 ± 0.2 at 25°C
Source:  This medium is available as a premixed powder from BD Di-
agnostic Systems and Oxoid Unipath.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes
or flasks. Autoclave for 15 min at 15 psi pressure–121°C.
Use: For the cultivation and maintenance of Eubacterium combesii,
Eubacterium contortum, and a variety of other anaerobic bacteria.