Schaedler HiVeg Broth

[所属分类:培养基配方] [发布时间:2021-8-12] [发布人:网站管理员2] [阅读次数:] [返回]

Schaedler HiVeg Broth

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Glucose.......................................................................................5.83g
Plant hydrolysate ........................................................................5.67g
Plant peptone No. 3.......................................................................5.0g
Tris(hydroxymethyl)aminomethane buffer...................................3.0g
Yeast extract..................................................................................5.0g
NaCl............................................................................................1.67g
Papaic digest of soybean meal......................................................1.0g
K 2 HPO 4 .......................................................................................0.83g
L -Cystine.......................................................................................0.4g
Fe 4 (P 2 O 7 ) 3 ·H 2 O ..........................................................................0.01g
Sheep blood, defibrinated........................................................50.0mL
pH 7.6 ± 0.2 at 25°C
Source: This medium, without blood, is available as a premixed pow-
der from HiMedia.
Preparation of Medium: Add components, except sheep blood, to
distilled/deionized water and bring volume to 950.0mL. Mix thorough-
ly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi
pressure–121°C. Cool to 45°–50°C. Aseptically add 50.0mL of sterile
sheep blood. Mix thoroughly. Aseptically distribute to tubes or flasks.
Use: For the enumeration of various aerobic and anaerobic bacterial
species present in the gastrointestinal tract.