Schneider’s Drosophila Medium

[所属分类:培养基配方] [发布时间:2021-8-12] [发布人:网站管理员2] [阅读次数:] [返回]

Schneider’s Drosophila Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

MgSO 4 ·7H 2 O................................................................................3.7g
NaCl..............................................................................................2.1g
Yeast extract..................................................................................2.0g
Trehalose.......................................................................................2.0g
D- Glucose......................................................................................2.0g
L- Glutamine ..................................................................................1.8g
L- Lysine·HCl.................................................................................1.7g
L- Proline........................................................................................1.7g
KCl................................................................................................1.6g
Na 2 HPO 4 ·7H 2 O.............................................................................1.3g
L- Glutamic acid.............................................................................0.8g
L- Methionine.................................................................................0.8g
CaCl 2 , anhydrous..........................................................................0.6g
KH 2 PO 4 .........................................................................................0.5g
β-Alanine......................................................................................0.5g
L- Tyrosine .....................................................................................0.5g
L- Arginine.....................................................................................0.4g
L- Aspartic acid..............................................................................0.4g
L- Histidine.....................................................................................0.4g
L- Threonine...................................................................................0.4g
NaHCO 3 ........................................................................................0.4g
Glycine..........................................................................................0.3g
L- Serine.........................................................................................0.3g
L- Valine.........................................................................................0.3g
L- Isoleucine...................................................................................0.2g
L- Leucine ......................................................................................0.2g
L- Phenylalanine.............................................................................0.2g
α-Ketoglutaric acid.......................................................................0.2g
Fumaric acid.................................................................................0.1g
Malic acid.....................................................................................0.1g
Succinic acid.................................................................................0.1g
L- Cystine.......................................................................................0.1g
L- Tryptophan.................................................................................0.1g
L- Cysteine ...................................................................................0.06g
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Filter sterilize.
Use: For the cultivation of Drosophila and other insect species. Also used
as a chemically defined supplement for the cultivation of fastidious micro-
organisms.