Schuster's Axenic Naegleria Medium

[所属分类:培养基配方] [发布时间:2021-8-12] [发布人:网站管理员2] [阅读次数:] [返回]

Schuster's Axenic Naegleria Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Peptone..........................................................................................2.5g
Yeast extract..................................................................................2.5g
Fetal bovine serum, inactivated.............................................100.0mL
Liver desiccate solution.........................................................100.0mL
Na 2 HPO 4 (0.05M)....................................................................60.0mL
KH 2 PO 4 (0.05M)......................................................................40.0mL
Liver Desiccate Solution:
Composition per 100.0mL:
Liver, desiccated ...........................................................................5.0g
Preparation of Liver Desiccate Solution : Add desiccated liver to
distilled/deionized water and bring volume to 100.0mL. Mix thoroughly.
Filter through Whatman no. 1 filter paper to remove sediment. Autoclave
filtrate for 15 min at 15 psi pressure–121°C.
Preparation of Medium: Add components, except fetal bovine se-
rum, to distilled/deionized water and bring volume to 900.0mL. Mix
thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Immedi-
ately prior to use, aseptically add 100.0mL of sterile fetal bovine se-
rum. Mix thoroughly. Aseptically distribute into sterile tubes or flasks.
Use: For the cultivation of Naegleria gruberi.