SCY Medium

[所属分类:培养基配方] [发布时间:2021-8-12] [发布人:网站管理员2] [阅读次数:] [返回]

SCY Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Sucrose..........................................................................................1.0g
Pancreatic digest of casein............................................................0.9g
NaCl............................................................................................0.05g
Papaic digest of soybean meal....................................................0.03g
K 2 HPO 4 .....................................................................................0.025g
Vitamin solution.......................................................................10.0mL
pH 7.1 ± 0.2 at 25°C
Vitamin Solution:
Composition per 10.0mL:
Thiamine....................................................................................0.4mg
Vitamin B 12 ..............................................................................0.01mg
Preparation of Vitamin Solution : Add components to distilled/
deionized water and bring volume to 10.0mL. Mix thoroughly. Filter
sterilize.
Preparation of Medium: Add components, except vitamin solu-
tion, to distilled/deionized water and bring volume to 990.0mL. Mix
thoroughly. Adjust pH to 7.1. Autoclave for 15 min at 15 psi pressure–
121°C. Aseptically add 10.0mL of sterile vitamin solution. Mix thor-
oughly. Aseptically distribute into sterile tubes or flasks.
Use: For the cultivation and maintenance of Flavobacterium species.