SCY Medium (Maintenance SCY Medium)

[所属分类:培养基配方] [发布时间:2021-8-12] [发布人:网站管理员2] [阅读次数:] [返回]

SCY Medium
(Maintenance SCY Medium)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Solution A.....................................................................................1.0L
Solution B..............................................................................200.0mL
Solution A:
Composition per liter:
Agar............................................................................................10.0g
Pancreatic digest of casein..........................................................0.92g
NaCl............................................................................................0.05g
Papaic digest of soybean meal....................................................0.03g
K 2 HPO 4 .....................................................................................0.025g
pH 7.0 ± 0.2 at 25°C
Preparation of Solution A: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Distribute into tubes in 10.0mL volumes. Autoclave for 15
min at 15 psi pressure–121°C. Allow tubes to cool in a slanted position.
Solution B:
Composition per 200.0mL:
Sucrose..........................................................................................2.0g
Yeast extract..................................................................................0.5g
Thiamine....................................................................................0.8mg
Vitamin B 12 ..............................................................................0.02mg
pH 8.5 ± 0.2 at 25°C
Preparation of Solution B: Add components to slightly alkaline
tap water, pH 8.5, and bring volume to 200.0mL. Mix thoroughly. Fil-
ter sterilize.
Preparation of Medium:  Inoculate bacteria onto prepared slants of
solution A. After inoculation of tubes, aseptically add 2.0mL of sterile
solution B on top of each slant.
Use:  For the cultivation and maintenance of iron bacteria. For the cul-
tivation and maintenance of Haliscomenobacter hydrossis.