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SDS HiVeg Agar with Polymyxin B (Sodium Dodecyl Sulfate Polymyxin Sucrose HiVeg Agar)
[所属分类:培养基配方] [发布时间:2021-8-12] [发布人:网站管理员2] [阅读次数:] [返回]
SDS HiVeg Agar with Polymyxin B
(Sodium Dodecyl Sulfate Polymyxin Sucrose HiVeg
Agar)
Agar............................................................................................15.0g
Sucrose........................................................................................15.0g
Plant peptone No. 3.....................................................................10.0g
Plant extract..................................................................................5.0g
Sodium dodecyl sulfate.................................................................1.0g
Bromthymol Blue.......................................................................0.04g
Cresol Red ..................................................................................0.04g
Polymyxin Bsolution.................................................................1.0mL
pH 7.6 ± 0.2 at 25°C
Source: This medium, without polymyxin B solution, is available as
a premixed powder from HiMedia.
Polymyxin B Solution:
Composition per 1.0mL:
Polymyxin B..............................................................................1.0mg
Preparation of Polymyxin B Solution: Add polymyxin B to dis-
tilled/deionized water and bring volume to 1.0mL. Mix thoroughly. Fil-
ter sterilize.
Preparation of Medium: Add components, except polymyxin B
solution, to distilled/deionized water and bring volume to 1.0L. Mix
thoroughly. Gently heat and bring to boiling. Distribute into tubes or
flasks. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–
50°C. Aseptically add 1.0mL of polymyxin B solution. Mix thorough-
ly. Pour into sterile Petri dishes.
Use: For the enrichment, isolation, and enumeration of Vibrio vulnifi-
cus from seafood.
(Sodium Dodecyl Sulfate Polymyxin Sucrose HiVeg
Agar)
山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com
Composition per liter:
NaCl............................................................................................20.0gAgar............................................................................................15.0g
Sucrose........................................................................................15.0g
Plant peptone No. 3.....................................................................10.0g
Plant extract..................................................................................5.0g
Sodium dodecyl sulfate.................................................................1.0g
Bromthymol Blue.......................................................................0.04g
Cresol Red ..................................................................................0.04g
Polymyxin Bsolution.................................................................1.0mL
pH 7.6 ± 0.2 at 25°C
Source: This medium, without polymyxin B solution, is available as
a premixed powder from HiMedia.
Polymyxin B Solution:
Composition per 1.0mL:
Polymyxin B..............................................................................1.0mg
Preparation of Polymyxin B Solution: Add polymyxin B to dis-
tilled/deionized water and bring volume to 1.0mL. Mix thoroughly. Fil-
ter sterilize.
Preparation of Medium: Add components, except polymyxin B
solution, to distilled/deionized water and bring volume to 1.0L. Mix
thoroughly. Gently heat and bring to boiling. Distribute into tubes or
flasks. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–
50°C. Aseptically add 1.0mL of polymyxin B solution. Mix thorough-
ly. Pour into sterile Petri dishes.
Use: For the enrichment, isolation, and enumeration of Vibrio vulnifi-
cus from seafood.
