Seawater Agar

[所属分类:培养基配方] [发布时间:2021-8-12] [发布人:网站管理员2] [阅读次数:] [返回]

Seawater Agar

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................20.0g
Beef extract.................................................................................10.0g
Peptone........................................................................................10.0g
Artificial seawater..................................................................750.0mL
Artificial Seawater
Composition per liter:
NaCl..........................................................................................28.13g
MgSO 4 ·7H 2 O................................................................................3.5g
MgCl 2 ..........................................................................................2.55g
CaCl 2 ............................................................................................1.2g
KCl..............................................................................................0.77g
NaHCO 3 ...................................................................................... 0.11g
pH 7.3 ± 0.2 at 25°C
Preparation of Artificial Seawater:  Natural seawater is stored in
the dark for at least 3 weeks to “age.” If natural seawater is not avail-
able, use artificial seawater. To prepare artificial seawater, add compo-
nents to distilled/deionized water and bring volume to 1.0L. Mix
thoroughly.
Preparation of Medium:  Add beef extract and peptone to distilled/
deionized water and bring volume to 250.0mL. Mix thoroughly. Adjust
pH to 7.8. Gently heat and bring to boiling. Boil for 10 min. Add
750.0mL of natural or artificial seawater. Mix thoroughly. Adjust pH
to 7.3. Add 20.0g of agar. Mix thoroughly. Gently heat and bring to
boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi
pressure–121°C. Pour into sterile Petri dishes or leave in tubes.
Use: For the cultivation and maintenance of Alteromonas rubra,
Brevibacterium stationis, Chromohalobacter marismortui, Flectoba-
cillus marinus, Marinococcus albus, Marinococcus halophilus, Pas-
teurella piscicida, Photobacterium phosphoreum, Planococcus cit-
reus, Planococcus kocurii, Vibrio adaptatus, Vibrio campbellii, Vibrio
costicola, Vibrio harveyi, Vibrio mediterranei, Vibrio natriegens, and
other Vibrio species.